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Amniotic Membrane Could Be Used to Repair Cartilage in Osteoarthritis
The results show that cryo-preserved HAM is useful as a scaffold for growing human chondrocytes in cell therapy and for repairing human cartilage injuries. “It provides a more regular surface and fills in the cavities and fissures”, explains Blanco.
Image: Osteoarthritis is characterized by impairment of cartilage and subchondral bone. In this image, an example of damaged knee cartilage is shown.
The authors cultivated the chondrocytes (cells that form part of the cartilaginous tissue), isolated from human articular cartilage, on the amniotic membrane over a period of three and four weeks. The amniotic membranes were used to develop 44 repair models of arthritic human articular cartilage in vitro, which was assessed between four and 16 weeks later.
The HAM also bonds well with the native cartilage. “In some models, we could not differentiate between where the native tissue stopped and the neo-synthesised tissue began”, says the expert. This tissue had a fibrous appearance and high cellular density (cellularity), which in some cases was greater than that of the actual native cartilage.
The use of differentiated chondrocytes is a useful therapeutic option for repairing articular cartilage injuries. However, there are limitations to implanting these cells, since many patients will be ruled out due to their lack of healthy chondrocytes, and this technique also causes additional damage to the joint.
“Transplanting chondrocytes cultivated on different natural or synthetic ‘scaffolds’ is used today in cell tissue engineering. The HAM has sparked great interest over recent years, above all in the field of regenerative medicine”, concludes Blanco.
Osteoarthritis (OA) is a major articular pathology that is characterised by alteration of the cartilage and the bone that supports it, the subchondral bone. As the current pharmacological and surgical osteoarthritis treatments have only palliative effects, cell therapy is a new clinical approach for repairing damaged or destroyed tissues.
HAM has many clinical advantages as a support – it is an anti-microbial, anti-angiogenic, anti-tumour tissue, which reduces inflammation and pain and improves scarring. In addition, the amnios of the HAM has no immune response, meaning there are no risks associated with transplanting it, and it contains many of the components of natural cartilage.
1. Francisco J. Blanco, et al. Potential use of the human amniotic membrane as a scaffold in human articular cartilage repair. Cell and Tissue Banking. DOI: 10.1007/s10561-009-9144-1