Cortical GABAergic Neurons

Cryopreserved Cortical GABAergic Neurons (BX-0400) were cultured in 96-well plates for three weeks and then loaded with Calbryte-520. Spontaneous oscillations were recorded in all wells simultaneously using an FDSS/µCell Functional dr-ug Screening System (Hamamatsu). Cortical GABAergic Neurons (BX-0400) were cultured on Axion Biosystems MEA plates for several weeks and recorded regularly. Below, a time course of the number of active electrodes, mean firing frequency, and synchrony index reveal the development of neuronal activity in Cortical GABAergic Neurons over several weeks in culture.